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GrainGenes Marker Report: Xgwm515

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Locus
Xgwm515
Type
Microsatellite
Chromosome
2A
Map
Ta-NxW-2A152.5
Wheat, UC1110 x PI610750 2A86.4
Wheat-Composite2004-2A56
[ Show Nearby Loci ]
Map Data
Wheat, Branched spike gene, 2009
Wheat, Composite, 2004
Wheat, Nanda2419 x Wangshuibai
Wheat, UC1110 x PI610750
Probe
WMS515
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
[ Hide all but 1 of 7 ]
Xgwm515-2A
Xgwm515-2D
Xgwm515.1-2A
Xgwm515b
gwm515
gwm515-2A
gwm515-2D



Probe for Locus
WMS515
Locus
Xgwm515-2A
[ Show all 8 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)17(TCAT)(GT)6
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AACACAATGGCAAATGCAGA 3'
5' CCTTCCTAGTAAGTGTGCCTCA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS515
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring