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GrainGenes Marker Report: Xgwm518

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Locus
Xgwm518
Type
Microsatellite
Chromosome
6B
Chromosome Arm
6BS
Map
Tt-LxG-6B76.9
[ Show Nearby Loci ]
Map Data
T. turgidum, Langdon x G18-16
Probe
WMS518
Possible Orthologs
[ Hide all but 1 of 7 ]
Xgwm518-6B
Xgwm518.1-6B
Xgwm518a
Xgwm518b
gwm518
gwm518-6B
gwm518-6D



Probe for Locus
WMS518
Locus
Xgwm518
[ Show all 8 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)34
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AATCACAACAAGGCGTGACA 3'
5' CAGGGTGGTGCATGCAT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS518
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring