Query (optional)   in Class  

GrainGenes Marker Report: Xgwm526-2R

[Submit comment/correction]

Locus
Xgwm526-2R
Type
Microsatellite
Chromosome
2R
Map
Secale-N7xN2-2004-2R163
[ Show Nearby Loci ]
Map Data
Rye, N7 x N2, 2004
Species
Secale cereale
Probe
WMS526
Reference
ReferenceKhlestkina EK et al. (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags Theoretical and Applied Genetics 109:725-732.
Possible Orthologs
Xgwm526
[ Show all 11 ]



Probe for Locus
WMS526
Locus
[ Hide all but 1 of 13 ]
Xgwm526
Xgwm526-2A
Xgwm526.2
Xgwm526.1
Xgwm526-2B
Yr7
Xgwm526.3-2B
Xgwm526.1-2D
Xgwm526-2R
gwm526
gwm526-2A
gwm526-2B
Xgwm526.2-2A
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)16
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' CAATAGTTCTGTGAGAGCTGCG 3'
5' CCAACCCAAATACACATTCTCA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS526
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring