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GrainGenes Marker Report: Xgwm533c

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Locus
Xgwm533c
Type
Microsatellite
Chromosome
3B
Map
Wheat-Composite2004-3B52
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMS533
Possible Orthologs
[ Hide all but 1 of 11 ]
gwm533
Xgwm533-3B
Xgwm533-3B.1
Xgwm533-3B.2
Xgwm533-3D
Xgwm533.1
Xgwm533.2
Xgwm533a
Xgwm533b
gwm533.1
gwm533-3B



Probe for Locus
WMS533
Locus
Xgwm533-3B
[ Show all 14 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)18(CA)20
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AAGGCGAATCAAACGGAATA 3'
5' GTTGCTTTAGGGGAAAAGCC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS533
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring