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GrainGenes Marker Report: Xgwm538

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Locus	Xgwm538
Type	Microsatellite
Chromosome	4B 4D
Chromosome Arm	4BL
Map	[ Hide all but 1 of 6 ] Ta-NxW-4B 52.2 Tt-LxG-4B 0 Wheat, Avalon x Cadenza 4B1 40.6 Wheat, UC1110 x PI610750 4B 57.8 Wheat-Composite2004-4B 56 Wheat-Composite2004-4D 125
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Map Data	T. turgidum, Langdon x G18-16 Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMS538
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm538-4B Xgwm538-5R gwm538 gwm538-4B
Probe for Locus	WMS538
Locus	Xgwm538 Xgwm538-4B Xgwm538-5R gwm538 gwm538-4B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)6(T)(GT)10 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GCATTTCGGGTGAACCC 3' 5' GTTGCATGTATACGTTAAGCGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS538
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring