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GrainGenes Marker Report: Xgwm540-4B

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Locus	Xgwm540-4B
Type	Microsatellite
Chromosome	4B
Chromosome Arm	4BL
Map	Kofa/UC1113-4B 39.9 Ta-SSR-2004-4B 21.7 Triticum-AxF-4B 6
	[ Show Nearby Loci ]
Map Data	Durum wheat, Kofa x UC1113 Wheat, Arina x Forno Wheat, Consensus SSR, 2004
Species	Triticum aestivum
Probe	WMS540
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs	[ Hide all but 1 of 6 ] Xgwm540 Xgwm540-5B Xgwm540a gwm540 gwm540-4B gwm540-5B
Probe for Locus	WMS540
Locus	Xgwm540-5B [ Show all 7 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)3(CC)(CT)16 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCTCGCTGTGAAATCCTATTTC 3' 5' AGGCATGGATAGAGGGGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS540
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring