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GrainGenes Marker Report: Xgwm544a

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Locus
Xgwm544a
Type
Microsatellite
Map
Svevo/Ciccio-5B44.3
[ Show Nearby Loci ]
Breakpoint Interval
5BS8-0.56-0.71
Map Data
Durum wheat, Svevo x Ciccio
Probe
WMS544
Possible Orthologs
Xgwm544
[ Show all 7 ]



Probe for Locus
WMS544
Locus
[ Hide all but 1 of 8 ]
Xgwm544-5B
Xgwm544
Xgwm544b
Xgwm544a
gwm544
gwm544-5B
Xgwm544a-5B
Xgwm544b-1B
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)12(ATCT)5(CT)16
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' TAGAATTCTTTATGGGGTCTGC 3'
5' AGGATTCCAATCCTTCAAAATT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS544
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring