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GrainGenes Marker Report: Xgwm582a

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Locus	Xgwm582a
Type	Microsatellite
Chromosome	6D
Map	Wheat-Composite2004-6D 94
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS582
Possible Orthologs	[ Hide all but 1 of 6 ] Xgwm582 Xgwm582-1B Xgwm582-6B Xgwm582-6D gwm582 gwm582-1B
Probe for Locus	WMS582
Locus	Xgwm582-1B [ Show all 8 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)27imp(TA)6 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AAGCACTACGAAAATATGAC 3' 5' TCTTAAGGGGTGTTATCATA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS582
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring