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GrainGenes Marker Report: Xgwm604-5B

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Locus	Xgwm604-5B
Type	Microsatellite
Chromosome	5B
Chromosome Arm	5BL
Map	Chinese_Spring_Deletion_SSR_5B 0.895 [ Show all 8 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	QGw1.inra-5B
Breakpoint Interval	5BL16-0.79-1.00
Map Data	[ Hide all but 1 of 8 ] T. turgidum, Messapia x dicoccoides, SSR T. turgidum, Omrabi5 x dicoccoides, QTL Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe	WMS604
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Possible Orthologs	Xgwm604 Xgwm604-1B gwm604 gwm604-5B
Probe for Locus	WMS604
Locus	Xgwm604-5B Xgwm604 Xgwm604-1B gwm604 gwm604-5B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)29 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TATATAGTTCAATATGACCCG 3' 5' ATCTTTTGAACCAAATGTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS604
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring