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GrainGenes Marker Report: Xgwm614

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Locus	Xgwm614
Type	Microsatellite
Chromosome	2A 2B
Chromosome Arm	2BS
Map	Tt-LxG-2B 0 Wheat, Avalon x Cadenza 2A 0 Wheat, UC1110 x PI610750 2B 0 Wheat-Composite2004-2A 10 Wheat-Composite2004-2B 3
	[ Show Nearby Loci ]
Breakpoint Interval	C-2AS5-0.87
Map Data	T. turgidum, Langdon x G18-16 Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMS614
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm614-2A [ Show all 14 ]
Probe for Locus	WMS614
Locus	[ Hide all but 1 of 16 ] Xgwm614 Xgwm614-2A Xgwm614-2D.1 Xgwm614-2D.2 Xgwm614-2B Xgwm614.2 Xgwm614.1 Xgwm614.1-2A Xgwm614.2-2B Xgwm614-4A Lr17a gwm614 gwm614-2A gwm614-2B Xgwm614.1-2D Xgwm614.2-2D
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)23imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GATCACATGCATGCGTCATG 3' 5' TTTTACCGTTCCGGCCTT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS614
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring