GrainGenes Marker Report: Xgwm666-5A
Locus
Xgwm666-5A
Type
Microsatellite
Chromosome
5A
Chromosome Arm
5AL
Map
Chinese_Spring_Deletion_SSR_5A 0.675
[ Show all 6 ]
[ Show Nearby Loci ]
Breakpoint Interval
5AL10-0.57-0.78
Map Data
[ Hide all but 1 of 6 ] T. turgidum, Messapia x dicoccoides, SSR Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, SSR
Species
Triticum aestivum
Probe
WMS666
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-5A, the intervals most likely to contain locus are Xbcd1235.1 - Xbcd183 or Xbcd183 - Xrz395
Possible Orthologs
Xgwm666
[ Show all 17 ]
Probe for Locus
WMS666
Locus
Xgwm666-3A
[ Show all 18 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)13 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GCACCCACATCTTCGACC 3'
5' TGCTGCTGGTCTCTGTGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS666
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm666-5A
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