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GrainGenes Marker Report: Xgwm95

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Locus
Xgwm95
Type
Microsatellite
Chromosome
2A
Chromosome Arm
2AS
Map
[ Hide all but 1 of 6 ]
Svevo/Ciccio-2A87.5
Ta-NxW-2A154.2
Tt-LxG-2A56
Wheat, Finch x Eltan 2A9.9
Wheat, UC1110 x PI610750 2A86.4
Wheat-Composite2004-2A56
[ Show Nearby Loci ]
Breakpoint Interval
C-2AS5-0.78
Map Data
Durum wheat, Svevo x Ciccio
[ Show all 6 ]
Probe
WMS95
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
Xgwm95-2A
[ Show all 10 ]



Probe for Locus
WMS95
Locus
Xgwm95
[ Show all 11 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (AC)16
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GATCAAACACACACCCCTCC 3'
5' AATGCAAAGTGAAAAACCCG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS95
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring