GrainGenes Marker Report: Xwmc112-2D
Locus
Xwmc112-2D
Type
Microsatellite
Chromosome
2D
Map
CS/SQ1-2D 21.4 Ta-SSR-2004-2D 28 Ta-Synthetic/Opata-GPW-2D 67.5 USG3209/Jaypee-2D 5.5 Wheat-2008-Corrigin-CS(T2D)-2D 32
[ Show Nearby Loci ]
Map Data
Wheat 2008, Corrigin x CS(T2D), C gene Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, USG3209 x Jaypee
Probe
WMC112
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc112 Xwmc112-3A Xwmc112y wmc112 wmc112-2D
Probe for Locus
WMC112
Locus
[ Hide all but 1 of 6 ] Xwmc112-2D Xwmc112 Xwmc112y Tg1 wmc112 wmc112-2D
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 8g8 Donor: Flore, Verneuil Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 251 bases located on 5B AGR170. Trimmed to Rsa1 at both ends. (CA)17 202 to 235, (CT)34 134 to 201. Good clear polymorphism (1 locus).
Type
PCR SSR
PCR primers
Forward TgAgTTgTggggTcTTgTTTgg at 105 length 22 bases, Tm 66.0
Reverse TgAAggAgggcAcATATcgTTg at 328 length 22 bases, Tm 66.0
SSR size
245 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC112
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc112-2D
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