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GrainGenes Marker Report: Xwmc149

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Locus	Xwmc149
Type	Microsatellite
Synonym	Other name XAWM149
Chromosome	2B 2D 5B
Map	Wheat, UC1110 x PI610750 5B 0.6 Wheat-Composite2004-2B 124 Wheat-Composite2004-2D 56 Wheat-Composite2004-5B 19
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMC149
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc149-2A [ Show all 16 ]
Probe for Locus	WMC149
Locus	[ Hide all but 1 of 17 ] Xwmc149a-5B Xwmc149-5B Xwmc149.1 Xwmc149.2 Xwmc149 Xwmc149-2A Xwmc149-2B wmc149 wmc149-5B wmc149a wmc149a-2B wmc149b wmc149b-2B wmc149-2A wmc149-2B.1 wmc149-2B.3 wmc149-2B.2
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11h1 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 180 bases located on 5B, 185 bases located on 1B, 230 bases located on 2B AGR204.8.SEQ. Trimmed to Rsa1 at both ends. (CT)8 50 to 65, (CT)24 68 to 115. (3-4 loci, 2-3 polymorphic).
Type	PCR SSR
PCR primers	Forward AcAgAcTTggTTggTgccgAgc at 6 length 22 bases, Tm 70 Reverse ATgggcgggggTgTAgAgTTTg at 214 length 22 bases, Tm 70
SSR size	230 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC149
Source Species	Triticum aestivum