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GrainGenes Marker Report: Xwmc149-5B

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Locus	Xwmc149-5B
Type	Microsatellite
Chromosome	5B
Chromosome Arm	5BS
Map	Kofa/UC1113-5B 15.1 Ta-SSR-2004-5B 39.3 Ta-Synthetic/Opata-GPW-5B 12.2 Ta-Synthetic/Opata-SSR2-5B 11
	[ Show Nearby Loci ]
Map Data	Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC149
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	[ Hide all but 1 of 16 ] Xwmc149 Xwmc149-2A Xwmc149-2B Xwmc149.1 Xwmc149.2 Xwmc149a-5B wmc149 wmc149b-2B wmc149a-2B wmc149-5B wmc149b wmc149a wmc149-2A wmc149-2B.1 wmc149-2B.3 wmc149-2B.2
Probe for Locus	WMC149
Locus	Xwmc149a-5B [ Show all 17 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11h1 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 180 bases located on 5B, 185 bases located on 1B, 230 bases located on 2B AGR204.8.SEQ. Trimmed to Rsa1 at both ends. (CT)8 50 to 65, (CT)24 68 to 115. (3-4 loci, 2-3 polymorphic).
Type	PCR SSR
PCR primers	Forward AcAgAcTTggTTggTgccgAgc at 6 length 22 bases, Tm 70 Reverse ATgggcgggggTgTAgAgTTTg at 214 length 22 bases, Tm 70
SSR size	230 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC149
Source Species	Triticum aestivum