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GrainGenes Marker Report: Xwmc206d

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Locus	Xwmc206d
Type	Microsatellite
Chromosome	4D
Map	Svevo/Ciccio-3A 95.6 Wheat-Composite2004-4D 72
	[ Show Nearby Loci ]
Breakpoint Interval	C-3AL3-0.42
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004
Probe	WMC206
Possible Orthologs	wmc206 [ Show all 20 ]
Probe for Locus	WMC206
Locus	[ Hide all but 1 of 21 ] Xwmc206-1B Xwmc206d Xwmc206b Xwmc206a Xwmc206.3 Xwmc206.1 Xwmc206-3B Xwmc206-4D Xwmc206-5D Xwmc206-6A wmc206 wmc206-4B Xwmc206a-5B Xwmc206b-4B Xwmc206c Xwmc206d-3A wmc206-1B wmc206-3B wmc206-4D wmc206-5D wmc206-6A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3g2 Donor: Jack, Plant Breeding International Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 190 bases located on 2B, 200 bases located on 2D Unilever comments '(estimated size = 700bp) sequence falls off after repeat in forward seq which is probably why I cant align them. ' Consensus shown is from reverse sequence only, forward sequence showed numerous ambiguities. Trimmed to insert at 5' end. Take care to use primers close in to the microsatellite on 5' side. (CA)29 332 to 389.
Type	PCR SSR
PCR primers	Forward TTgTgcTcgTgAATTgcATAcc at 283 length 22 bases, Tm 65 Reverse gccAAAATggcAgcTTcTcTTA at 493 length 22 bases, Tm 64
SSR size	232 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC206
Source Species	Triticum aestivum