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GrainGenes Marker Report: Xwmc216-1B

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Locus
Xwmc216-1B
Type
Microsatellite
Chromosome
1B
Chromosome Arm
1BL
Map
Ta-SSR-2004-1B35.1
Triticum-AxF-1B57
[ Show Nearby Loci ]
Map Data
Wheat, Arina x Forno
Wheat, Consensus SSR, 2004
Species
Triticum aestivum
Probe
WMC216
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
ReferencePaillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs
[ Hide all but 1 of 7 ]
wmc216-1D
Xwmc216
Xwmc216-1D
Xwmc216.1
Xwmc216.3eb
wmc216
wmc216-1B



Probe for Locus
WMC216
Locus
Xwmc216-1D
[ Show all 8 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11h7
Donor: Benoist
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 128 bases located on 1D
AGR205.8. Trimed to insert at 5' end and to Rsa1 at 3' end. (GT)22 42 to 85.
Type
PCR
SSR
PCR primers
Forward AcgTATccAgAcAcTgTggTAA at 13 length 22 bases, Tm 59
Reverse TAATggTggATccATgATAgcc at 114 length 22 bases, Tm 63
SSR size
123 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC216
Source Species
Triticum aestivum