Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc219

[Submit comment/correction]

Locus	Xwmc219
Type	Microsatellite
Chromosome	4A
Map	Svevo/Ciccio-4A 114 Ta-NxW-4A 161.6 Wheat-Composite2004-4A 140
	[ Show Nearby Loci ]
Breakpoint Interval	4AL4-0.80-1.00
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMC219
Possible Orthologs	Xwmc219-4A wmc219 wmc219-4A
Probe for Locus	WMC219
Locus	[ Hide all but 1 of 7 ] Xwmc219-4A Xwmc219 Stb12 Yr60 SrND643 wmc219 wmc219-4A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr60 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11b9 Donor: Benoist AGR205.18. Overlap of forward and reverse is hypothetical - both end within a substantial micorsatellite. The fragment sizes for the PCR from this sequence will not match the expected size. Trimmed to Rsa1 at both ends. (CA)57 316 to 429.
Type	PCR SSR
PCR primers	Forward TgcTAgTTTgTcATccgggcgA at 264 length 22 bases, Tm 71 Reverse cAATcccgTTcTAcAAgTTccA at 446 length 22 bases, Tm 63
SSR size	~204 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC219
Source Gene	Yr60 (Triticum)
Source Species	Triticum aestivum