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GrainGenes Marker Report: Xwmc243

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Locus	Xwmc243
Type	Microsatellite
Chromosome	2B 2D
Map	Ta-NxW-2B 39.1 Wheat, Avalon x Cadenza 2B 38.4 Wheat, UC1110 x PI610750 2B 28.9 Wheat-Composite2004-2B 28 Wheat-Composite2004-2D 66
	[ Show Nearby Loci ]
Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC243
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	[ Hide all but 1 of 11 ] Xwmc243-2B Xwmc243-2D Xwmc243-6A Xwmc243a-2B Xwmc243b Xwmc243b-7D wmc243-2B wmc243c wmc243 wmc243-2D wmc243-6A
Probe for Locus	WMC243
Locus	Xwmc243a-2B [ Show all 12 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5e4 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 158 bases located on 7D, 165 bases located on 2B Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)15 39 to 68. Small common sequence with 11D6 avoided.
Type	PCR SSR
PCR primers	Forward cgTcATTTccTcAAAcAcAccT at 8 length 22 bases, Tm 63 Reverse AccggcAgATgTTgAcAATAgT at 163 length 22 bases, Tm 62
SSR size	177 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC243
Source Species	Triticum aestivum