GrainGenes Marker Report: Xwmc243-2B
Locus
Xwmc243-2B
Type
Microsatellite
Chromosome
2B
Chromosome Arm
2BS
Map
Louise/Penawawa-2B 113.5 Ta-SSR-2004-2B 30 Ta-Synthetic/Opata-SSR2-2B 37.2
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, Synthetic x Opata, SSR 2
Probe
WMC243
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 11 ] Xwmc243 Xwmc243-2D Xwmc243-6A Xwmc243a-2B Xwmc243b Xwmc243b-7D wmc243-2B wmc243c wmc243 wmc243-2D wmc243-6A
Probe for Locus
WMC243
Locus
Xwmc243a-2B
[ Show all 12 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5e4 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 158 bases located on 7D, 165 bases located on 2B Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)15 39 to 68. Small common sequence with 11D6 avoided.
Type
PCR SSR
PCR primers
Forward cgTcATTTccTcAAAcAcAccT at 8 length 22 bases, Tm 63
Reverse AccggcAgATgTTgAcAATAgT at 163 length 22 bases, Tm 62
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC243
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc243-2B
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