GrainGenes Marker Report: Xwmc254
Locus
Xwmc254
Type
Microsatellite
Synonym
Other name XAWM254
Chromosome
1A 4B
Map
Svevo/Ciccio-1A 193.3 Wheat-Composite2004-1A 51 Wheat-Composite2004-4B 31
[ Show Nearby Loci ]
Breakpoint Interval
1AL3-0.61-1.00
Map Data
Durum wheat, Svevo x Ciccio Wheat, Composite, 2004
Probe
WMC254
Possible Orthologs
[ Hide all but 1 of 7 ] Xwmc254-1A Xwmc254-4B Xwmc254-6A wmc254 wmc254-1A wmc254-6A wmc254-4B
Probe for Locus
WMC254
Locus
Xwmc254-1A
[ Show all 8 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4b2 Donor: Gupta, Meerut No trimming done to sequence. (CA)28 31 to 86. Forward primer is not ideal.
Type
PCR SSR
PCR primers
Forward AgTAATcTggTccTcTcTTcTTcT at 2 length 24 bases, Tm 58
Reverse AggTAATcTccgAgTgcAcTTcAT at 171 length 24 bases, Tm 64
SSR size
193 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC254
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc254
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