GrainGenes Marker Report: Xwmc256-6A
Locus
Xwmc256-6A
Type
Microsatellite
Chromosome
6A
Chromosome Arm
6AL
Map
[ Hide all but 1 of 7 ] Chinese_Spring_Deletion_SSR_6A 0.725 CS/SQ1-6A 131.9 Kofa/UC1113-6A 57.4 Louise/Penawawa-6A 77.9 Ta-SSR-2004-6A 40.4 Ta-Synthetic/Opata-GPW-6A 89.9 Ta-Synthetic/Opata-SSR2-6A 87.4
[ Show Nearby Loci ]
Breakpoint Interval
6AL4-0.55-0.90
Map Data
Durum wheat, Kofa x UC1113
[ Show all 7 ]
Probe
WMC256
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc256 wmc256-6A wmc256
Probe for Locus
WMC256
Locus
Xwmc256-6A Xwmc256 wmc256-6A wmc256
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4h2.1 Donor: Gupta, Meerut Small linker like sequence at 234-241 in original ie. multiple inserts (2) in this sequence, this record shows first insert only (up to but excluding linker). No trimming done to sequence at 5'. (CA)12 65 to 88.
Type
PCR SSR
PCR primers
Forward ccAAATcTTcgAAcAAgAAccc at 37 length 22 bases, Tm 64
Reverse AccgATcgATggTgTATAcTgA at 132 length 22 bases, Tm 61
SSR size
117 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC256
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc256-6A
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