Query (optional)   in Class  

GrainGenes Marker Report: Xwmc256-6A

[Submit comment/correction]

Locus
Xwmc256-6A
Type
Microsatellite
Chromosome
6A
Chromosome Arm
6AL
Map
Chinese_Spring_Deletion_SSR_6A0.725
[ Show all 7 ]
[ Show Nearby Loci ]
Breakpoint Interval
6AL4-0.55-0.90
Map Data
[ Hide all but 1 of 7 ]
Durum wheat, Kofa x UC1113
Wheat, Chinese Spring x SQ1
Wheat, Consensus SSR, 2004
Wheat, Louise x Penawawa
Wheat, Physical, SSR
Wheat, Synthetic x Opata, GPW
Wheat, Synthetic x Opata, SSR 2
Probe
WMC256
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc256
wmc256-6A
wmc256



Probe for Locus
WMC256
Locus
Xwmc256-6A
Xwmc256
wmc256-6A
wmc256
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4h2.1
Donor: Gupta, Meerut
Small linker like sequence at 234-241 in original ie. multiple inserts (2) in this sequence, this record shows first insert only (up to but excluding linker). No trimming done to sequence at 5'. (CA)12 65 to 88.
Type
PCR
SSR
PCR primers
Forward ccAAATcTTcgAAcAAgAAccc at 37 length 22 bases, Tm 64
Reverse AccgATcgATggTgTATAcTgA at 132 length 22 bases, Tm 61
SSR size
117 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC256
Source Species
Triticum aestivum