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GrainGenes Marker Report: Xwmc264-5D

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Locus
Xwmc264-5D
Type
Microsatellite
Chromosome
5D
Map
Ta-SSR-2004-5D65.3
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC264
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc264
[ Show all 8 ]



Probe for Locus
WMC264
Locus
[ Hide all but 1 of 9 ]
Xwmc264-3A
Xwmc264.2
Xwmc264.1
Xwmc264
Xwmc264-5D
Xwmc264-2A
wmc264
wmc264-3A
wmc264-5D
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4c6
Donor: Gupta, Meerut
No trimming done to sequence. (GA)16 81 to 112.
Type
PCR
SSR
PCR primers
Forward cTccATcTATTgAgcgAAggTT at 55 length 22 bases, Tm 61
Reverse cAAgATgAAgcTcATgcAAgTg at 166 length 22 bases, Tm 63
SSR size
133 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC264
Source Species
Triticum aestivum