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GrainGenes Marker Report: Xwmc27-2B

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Locus
Xwmc27-2B
Type
Microsatellite
Chromosome
2B
Map
Ta-SSR-2004-2B60.4
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC27
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 6 ]
Xwmc27
Xwmc27-5B
Xwmc27.1
Xwmc27.2
wmc27
wmc27-2B



Probe for Locus
WMC27
Locus
Xwmc27.2
[ Show all 7 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2f9
Donor: Wang, Utah
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 352 bases located on 2D, 398 bases located on 2B
(GT)25 153 to 202.
Type
PCR
SSR
PCR primers
Forward AATAgAAAcAggTcAccATccg at 37 length 22 bases, Tm 62
Reverse TAgAgcTggAgTAgggccAAAg at 404 length 22 bases, Tm 64
SSR size
389 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC27
Source Species
Triticum aestivum