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GrainGenes Marker Report: Xwmc273c

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Locus	Xwmc273c
Type	Microsatellite
Chromosome	7B
Map	Wheat-Composite2004-7B 89
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC273
Possible Orthologs	Xwmc273-7A [ Show all 17 ]
Probe for Locus	WMC273
Locus	[ Hide all but 1 of 18 ] Xwmc273.3-7A Xwmc273.2-7B Xwmc273.1-7D Xwmc273-7B Xwmc273-7A Xwmc273.1 Xwmc273.2 Xwmc273c Xwmc273-7D wmc273 wmc273-7B wmc273a wmc273a-7A wmc273b wmc273b-7A Xwmc273a wmc273-7A.1 wmc273-7A.2
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3g8 Donor: Keller, Zurich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 182 bases located on 7B, 5D, 199 bases located on 7D, 5D, 1A, 235 bases located on 7A, 5D No trimming done to sequence. (GT)8 395 to 410, (GT)13 453 to 478.
Type	PCR SSR
PCR primers	Forward AgTTATgTATTcTcTcgAgccTg at 323 length 23 bases, Tm 58 Reverse ggTAAccAcTAgAgTATgTccTT at 479 length 22 bases, Tm 55
SSR size	179 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC273
Source Species	Triticum aestivum