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GrainGenes Marker Report: Xwmc276

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Locus	Xwmc276
Type	Microsatellite
Chromosome	7B
Map	Wheat, Finch x Eltan 7B.1 11.9 Wheat-Composite2004-7B 108
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Finch x Eltan
Probe	WMC276
Possible Orthologs	[ Hide all but 1 of 10 ] Xwmc276-7B wmc276 wmc276b-7B wmc276a-7B wmc276b wmc276a wmc276-7B wmc276-7B.1 wmc276-7B.2 wmc276-7B.3
Probe for Locus	WMC276
Locus	Xwmc276-7B [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3e9.2 Donor: Keller, Zurich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 283-306 bases located on 7B Second insert in 3e9. (CA)19 203 to 240, (CA)13 973 to 998, (CA)9 1000 to 1017, (CA)23 1026 to 1071. Primers shown are for second insert (coordinates refer to total insert).
Type	PCR SSR
PCR primers	Forward gAcATgTgcAccAgAATAgc at 817 length 20 bases, Tm 59 Reverse AgAAgAAcTATTcgAcTccT at 1089 length 20 bases, Tm 52
SSR size	292 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC276
Source Species	Triticum aestivum