GrainGenes Marker Report: Xwmc289-5D
Locus
Xwmc289-5D
Type
Microsatellite
Chromosome
5D
Map
Ta-SSR-2004-5D 60.2
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC289
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 12 ] Xwmc289 Xwmc289-5B Xwmc289a Xwmc289b wmc289 wmc289_1 wmc289_2 wmc289-5B wmc289-5B.1 wmc289-5B.2 wmc289-5D.1 wmc289-5D.2
Probe for Locus
WMC289
Locus
Xwmc289
[ Show all 13 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 13d2 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 164 bases located on 5D, 198 bases located on 5B Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)13 221 to 246, (CT)10 178 to 197.
Type
PCR SSR
PCR primers
Forward cATATgcATgcTATgcTggcTA at 146 length 22 bases, Tm 63
Reverse AgccTTTcAAATccATccAcTg at 324 length 22 bases, Tm 64
SSR size
200 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence
WMC289
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc289-5D
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