GrainGenes Marker Report: Xwmc313-4A
Locus
Xwmc313-4A
Type
Microsatellite
Chromosome
4A
Map
Conan/Reeder-4A 181.927 CS/SQ1-4A 29.2 Kofa/UC1113-4A 129.8 Ta-SSR-2004-4A 82.9
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Chinese Spring x SQ1 Wheat, Conan x Reeder Wheat, Consensus SSR, 2004
Probe
WMC313
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc313 wmc313 wmc313-4A
Probe for Locus
WMC313
Locus
[ Hide all but 1 of 6 ] Xwmc313-4A Xwmc313 Stb7 Yr60 wmc313 wmc313-4A
Reference
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 14f2 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 198 bases located on 4A Trimmed to Rsa1 at both ends. (CA)18 166 to 201.
Type
PCR SSR
PCR primers
Forward gcAgTcTAATTATcTgcTggcg at 78 length 22 bases, Tm 63
Reverse gggTccTTgTcTAcTcATgTcT at 253 length 22 bases, Tm 59
SSR size
197 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide (several loci).
Sequence
WMC313
Source Gene
Yr60 (Triticum)
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc313-4A
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