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GrainGenes Marker Report: Xwmc326

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Locus	Xwmc326
Type	Microsatellite
Chromosome	3B
Map	Ta-NxW-3B 204.1 Wheat, Avalon x Cadenza 3B 180.4 Wheat, UC1110 x PI610750 3B 81 Wheat-Composite2004-3B 74
	[ Show Nearby Loci ]
Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC326
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	[ Hide all but 1 of 10 ] Xwmc326-3A Xwmc326-3B Xwmc326-5B Xwmc326a Xwmc326a-1B Xwmc326c Xwmc326c-2A wmc326 wmc326-3B wmc326-5B
Probe for Locus	WMC326
Locus	Xwmc326-3B [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17d9 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 188 bases located on 3B Trimmed to insert at 5' end, and to Ssp1 at 3' end. (CT)16 112 to 143.
Type	PCR SSR
PCR primers	Forward ggAgcATcgcAggAcAgA at 22 length 18 bases, Tm 63 Reverse ggAcgAggAcgccTgAAT at 190 length 18 bases, Tm 63
SSR size	186 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC326
Source Species	Triticum aestivum