Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc332

[Submit comment/correction]

Locus	Xwmc332
Type	Microsatellite
Chromosome	2B
Map	Svevo/Ciccio-2B 152.8 Wheat, WH542 x Synthetic 2B 72.9 Wheat-Composite2004-2B 102
	[ Show Nearby Loci ]
Breakpoint Interval	2BL6-0.89-1.00
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004 Wheat, WH542 x Synthetic
Probe	WMC332
Possible Orthologs	Xwmc332-2B wmc332-2B wmc332
Probe for Locus	WMC332
Locus	[ Hide all but 1 of 6 ] Xwmc332-2B Xwmc332 wmc332-2B Sr28 wmc332 WMC332
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17b12 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2B Trimed to insert at both ends. (CT)12 136 to 159.
Type	PCR SSR
PCR primers	Forward cATTTAcAAAgcgcATgAAgcc at 51 length 22 bases, Tm 65 Reverse gAAAAcTTTgggAAcAAgAgcA at 198 length 22 bases, Tm 62
SSR size	169 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC332
Source Species	Triticum aestivum