GrainGenes Marker Report: Xwmc415b-5A
Locus
Xwmc415b-5A
Type
Microsatellite
Map Data
Durum wheat, Svevo x Ciccio
Probe
WMC415
Possible Orthologs
[ Hide all but 1 of 18 ] Xwmc415 Xwmc415-5A Xwmc415-5B Xwmc415a Xwmc415a-5B Xwmc415b wmc415 wmc415a-5A wmc415-5B wmc415_2 wmc415a wmc415_1 wmc415-5A wmc415-5A.1 wmc415-5A.2 wmc415-5A.3 wmc415-5B.1 wmc415-5B.2
Probe for Locus
WMC415
Locus
Xwmc415a
[ Show all 19 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 23e1 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 130 bases located on 5B, 142 bases located on 5A, 168 bases located on 5B Trimmed to Ssp1 at both ends of insert. (CA)23 271 to 316.
Type
PCR SSR
PCR primers
Forward AATTcgATAccTcTcAcTcAcg at 184 length 22 bases, Tm 60
Reverse TcAAcTgcTAcAAccTAgAccc at 323 length 22 bases, Tm 60
SSR size
161 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide;
Sequence
WMC415
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc415b-5A
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