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GrainGenes Marker Report: Xwmc422-7A

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Locus
Xwmc422-7A
Type
Microsatellite
Chromosome
7A
Map
CS/SQ1-7A115.9
Ta-SSR-2004-7A72.5
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Wheat, Consensus SSR, 2004
Probe
WMC422
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc422
wmc422
wmc422-7A



Probe for Locus
WMC422
Locus
[ Hide all but 1 of 6 ]
Xwmc422-7A
Xwmc422
Xwmc426b
Xwmc426a
wmc422
wmc422-7A
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 21h3
Donor: Langridge, CRC Australia
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 305 bases located on 7A
No trimming done to sequence at Agrogene, ends not located. (CT)16 25 to 56.
Type
PCR
SSR
PCR primers
Forward ggAcTAcTgAAcTggAgAgTgTg at 2 length 23 bases, Tm 60
Reverse gcATTAgAATTTggAgTTTggAg at 283 length 23 bases, Tm 61
SSR size
304 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (295-310 bases)
Sequence
WMC422
Source Species
Triticum aestivum