GrainGenes Marker Report: Xwmc445.c-3A
Locus
Xwmc445.c-3A
Type
Microsatellite
Map
Conan/Reeder-3A 30.1615
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder
Probe
WMC445
Possible Orthologs
Xwmc445
[ Show all 9 ]
Probe for Locus
WMC445
Locus
[ Hide all but 1 of 10 ] Xwmc445.3 Xwmc445.2 Xwmc445.1 Xwmc445 Xwmc445-5A wmc445-2B wmc445 wmc445-2A Xwmc445.c-3A wmc445-5A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 24g7 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 229 bases located on 2B Trimmed to insert at both ends. (GGC)6 182 to 199.
Type
PCR SSR
PCR primers
Forward AgAATAggTTcTTgggccAgTc at 38 length 22 bases, Tm 62
Reverse gAgATgATcTccTccATcAgcA at 245 length 22 bases, Tm 63
SSR size
229 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product. tested 51C anneal, no product;
Sequence
WMC445
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc445.c-3A
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