GrainGenes Marker Report: Xwmc453b-2B
Locus
Xwmc453b-2B
Type
Microsatellite
Map
CS/SQ1-2B 96.7
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Probe
WMC453
Possible Orthologs
[ Hide all but 1 of 12 ] Xwmc453 Xwmc453-2A Xwmc453-2D Xwmc453.1-2B Xwmc453.2-2A Xwmc453a Xwmc453a-2A Xwmc453b Xwmc453d-2D wmc453 wmc453-2A wmc453-2D
Probe for Locus
WMC453
Locus
Xwmc453a-2A
[ Show all 13 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2c2 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2A Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)35 119 to 188.
Type
PCR SSR
PCR primers
Forward AcTTgTgTccATAAccgAccTT at 73 length 22 bases, Tm 61
Reverse ATcTTTTgAggTTAcAAcccgA at 238 length 22 bases, Tm 61
SSR size
187 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (though complex).
Sequence
WMC453
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc453b-2B
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