GrainGenes Marker Report: Xwmc471
Locus
Xwmc471
Type
Microsatellite
Chromosome
3B
Map
Wheat-Composite2004-3B 45
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMC471
Possible Orthologs
[ Hide all but 1 of 7 ] wmc471 Xwmc471-3B Xwmc471-7B Xwmc471b wmc471-4B wmc471-3B wmc471-7B
Probe for Locus
WMC471
Locus
Xwmc471
[ Show all 8 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25e1 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 161 bases located on 4A Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)4 112 to 119, (GT)8 179 to 194, (GT)14 197 to 224.
Type
PCR SSR
PCR primers
Forward ggcAATAATAgTgcAAggAATg at 45 length 22 bases, Tm 60
Reverse gccgATAATgggcAATATAAgT at 235 length 22 bases, Tm 61
SSR size
212 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product. 51C anneal, polymorphic on acrylamide.
Sequence
WMC471
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc471
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