GrainGenes Marker Report: Xwmc473-4D
Locus
Xwmc473-4D
Type
Microsatellite
Chromosome
4D
Map
Conan/Reeder-4D 164.25 CS/SQ1-4D 19 Ta-SSR-2004-4D 32.5
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Conan x Reeder Wheat, Consensus SSR, 2004
Probe
WMC473
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 8 ] Xwmc473 Xwmc473-6B Xwmc473-7D Xwmc473a Xwmc473c wmc473 wmc473-6B wmc473-4D
Probe for Locus
WMC473
Locus
Xwmc473-4D
[ Show all 9 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25e2 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 111 bases located on 1D, 149 bases located on 4D Trimmed to Ssp1 at 5' end and to insert at 3' end. (CA)27 40 to 93.
Type
PCR SSR
PCR primers
Forward TcTgTTgcgcgAAAcAgAATAg at 6 length 22 bases, Tm 64
Reverse cccATTggAcAAcAcTTTcAcc at 122 length 22 bases, Tm 65
SSR size
138 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product. tested 51C anneal, no product.
Sequence
WMC473
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc473-4D
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