Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc474

[Submit comment/correction]

Locus	Xwmc474
Type	Microsatellite
Map	Ta-NxW-2B 117.8 Wheat, UC1110 x PI610750 2B 80.6
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC474
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc474-2A [ Show all 6 ]
Probe for Locus	WMC474
Locus	[ Hide all but 1 of 7 ] Xwmc474-2B Xwmc474 Xwmc474b Xwmc474-2A wmc474 wmc474-2B wmc474-2A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e3 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 2B Trimmed to insert at both ends. (GT)20 53 to 92.
Type	PCR SSR
PCR primers	Forward ATgcTATTAAAcTAgcATgTgTcg at 3 length 24 bases, Tm 60 Reverse AgTggAAAcATcATTccTggTA at 103 length 22 bases, Tm 60
SSR size	120 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product. 51C anneal, product.
Sequence	WMC474
Source Species	Triticum aestivum