GrainGenes Marker Report: Xwmc492
Locus
Xwmc492
Type
Microsatellite
Map
Ta-NxW-5A 70.1
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMC492
Possible Orthologs
Xwmc492-3A
[ Show all 6 ]
Probe for Locus
WMC492
Locus
[ Hide all but 1 of 7 ] Xwmc492-3D Xwmc492 Xwmc492-5A Xwmc492-3A wmc492-3D wmc492 wmc492-5A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25e9 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 150 bases located on 3D, 337 bases located on 7B, 7D, 450 bases located on 4D Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 5 to 29 (complement) show homology to 13A1 bases 164 to 188 (25 in longest run, maxScore = 31). (CA)8 260 to 275, (CA)8 278 to 293, (CA)9 296 to 313. Primers share a motif, small inverted repeats near the microsatellite.
Type
PCR SSR
PCR primers
Forward AggATcAgAATAgTgcTAccc at 192 length 21 bases, Tm 57
Reverse ATcccgTgATcAgAATAgTgT at 317 length 21 bases, Tm 58
SSR size
146 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (126-154 bases).
Sequence
WMC492
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc492
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