GrainGenes Marker Report: Xwmc497.2
Locus
Xwmc497.2
Type
Microsatellite
Map
Ta-NxW-4A 182.8
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMC497
Possible Orthologs
[ Hide all but 1 of 6 ] Xwmc497-4A Xwmc497-7A Xwmc497.1 Xwmc497b wmc497 wmc497-4A
Probe for Locus
WMC497
Locus
Xwmc497.2
[ Show all 7 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25a11 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 222 bases located on 4A, 250 bases located on 7D Trimmed to insert at both ends. (GT)22 262 to 305.
Type
PCR SSR
PCR primers
Forward cccgTggTTTTcTTTccTTcT at 109 length 21 bases, Tm 63
Reverse AAcgAcAgggATgAAAAgcAA at 307 length 21 bases, Tm 64
SSR size
219 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (210-220 bases).
Sequence
WMC497
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc497.2
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