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GrainGenes Marker Report: Xwmc500c

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Locus	Xwmc500c
Type	Microsatellite
Chromosome	1B
Map	Svevo/Ciccio-1B 23.6 Wheat-Composite2004-1B 28
	[ Show Nearby Loci ]
Breakpoint Interval	1BS.sat-0.31
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004
Probe	WMC500
Possible Orthologs	wmc500 [ Show all 26 ]
Probe for Locus	WMC500
Locus	[ Hide all but 1 of 28 ] Xwmc500-3B Xwmc500c Xwmc500a Xwmc500b Xwmc500g Xwmc500-1B.1 Xwmc500-1B.2 Xwmc500-4A Xwmc500-5B Xwmc500-2B Xwmc500-7B Stb14 wmc500 wmc500-3B wmc500-4A wmc500a wmc500a-1B wmc500b wmc500b-1B Xwmc500a-4A Xwmc500b-7B Xwmc500c-1B wmc500-1B wmc500-1B.1 wmc500-1B.2 wmc500-2B wmc500-5B wmc500-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25b12 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 155 bases located on 2B, 165 bases located on 7B, 180 bases located on 1B, 188 bases located on 3B, 204 bases located on 4A Trimmed to insert at both ends. (CA)13 38 to 63, (CA)10 66 to 85.
Type	PCR SSR
PCR primers	Forward ATAgcATgTTggAAcAgAgcAc at 15 length 22 bases, Tm 61 Reverse cTTAgATgcAAcTcTATgcggT at 178 length 22 bases, Tm 60
SSR size	185 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (polymorphism is superimposed on smear, 150-190 bases, complex).
Sequence	WMC500
Source Species	Triticum aestivum