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GrainGenes Marker Report: Xwmc505-3B

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Locus
Xwmc505-3B
Type
Microsatellite
Chromosome
3B
Map
Ta-SSR-2004-3B57.3
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC505
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 10 ]
wmc505
Xwmc505-3A
Xwmc505-3D
Xwmc505.1
Xwmc505.2
Xwmc505a
Xwmc505d
wmc505-3A
wmc505-3B
Xwmc505



Probe for Locus
WMC505
Locus
Xwmc505-3A
[ Show all 11 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 20g8
Donor: Byrne, Colorado State
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 115 bases located on 3A, 133 bases located on 3B, 90 bases located on 3D, 98 bases located on 3B
No trimming done to sequence at Agrogene, ends not located. (CA)31 55 to 116.
Type
PCR
SSR
PCR primers
Forward AggggAggAAAAccTTgTAATc at 28 length 22 bases, Tm 61
Reverse AcgAccTAcgTggTAgTTcTTg at 131 length 22 bases, Tm 60
SSR size
125 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (several loci) .
Sequence
WMC505
Source Species
Triticum aestivum