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GrainGenes Marker Report: Xwmc527-3B

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Locus	Xwmc527-3B
Type	Microsatellite
Chromosome	3B
Map	Ta-SSR-2004-3B 71.2
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004
Probe	WMC527
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	wmc527 [ Show all 9 ]
Probe for Locus	WMC527
Locus	[ Hide all but 1 of 10 ] Xwmc527 Xwmc527b Xwmc527a Xwmc527-3A Xwmc527-3B wmc527 wmc527-3B Xwmc527a-3B Xwmc527b-3A wmc527-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24B10 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 378 bases located on 3B Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)15 328 to 357, (GA)20 30 to 69.
Type	PCR SSR
PCR primers	Forward AcccAAgATTggTTgcAgAA at 4 length 20 bases, Tm 63 Reverse gcTAcAgAAAAccggAgccTAT at 366 length 22 bases, Tm 62
SSR size	386 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (360 bases).
Sequence	WMC527
Source Species	Triticum aestivum