GrainGenes Marker Report: Xwmc533-3D
Locus
Xwmc533-3D
Type
Microsatellite
Chromosome
3D
Map
Conan/Reeder-3D 4.38551 CS/SQ1-3D 71.3 Ta-SSR-2004-3D 32.8
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Conan x Reeder Wheat, Consensus SSR, 2004
Probe
WMC533
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc533 Xwmc533 Xwmc533-3B Xwmc533a wmc533-3B
Probe for Locus
WMC533
Locus
[ Hide all but 1 of 6 ] Xwmc533-3D Xwmc533 Xwmc533a Xwmc533-3B wmc533 wmc533-3B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 26h1 Donor: Madre, Secobra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 134 bases located on 3B, 152 bases located on 3D Trimmed to insert at both ends. (CA)20 189 to 228.
Type
PCR SSR
PCR primers
Forward AATTggATcggcAgTTggAg at 131 length 20 bases, Tm 64
Reverse AgcAAgcAgAgcATTgcgTT at 258 length 20 bases, Tm 65
SSR size
147 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (140-156 bases).
Sequence
WMC533
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc533-3D
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