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GrainGenes Marker Report: Xwmc552.1

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Locus
Xwmc552.1
Type
Microsatellite
Map
Ta-NxW-3D147.7
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMC552
Possible Orthologs
Xwmc552-3D
[ Show all 6 ]



Probe for Locus
WMC552
Locus
[ Hide all but 1 of 7 ]
Xwmc552-3D
Xwmc552.3
Xwmc552.2
Xwmc552.1
wmc552
Xwmc552
wmc552-3D
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 26e10
Donor: Momont
Trimmed to insert at both ends. (GT)25 91 to 140.
Type
PCR
SSR
PCR primers
Forward AcTAAggAgTgTgAgggcTgTg at 33 length 22 bases, Tm 62
Reverse cTcTcgcgcTATAAAAgAAggA at 160 length 22 bases, Tm 62
SSR size
149 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (140-160 bases).
Sequence
WMC552
Source Species
Triticum aestivum