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GrainGenes Marker Report: Xwmc59

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Locus	Xwmc59
Type	Microsatellite
Chromosome	1A
Map	Wheat, UC1110 x PI610750 1A 110.8 Wheat-Composite2004-1A 113
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMC59
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	[ Hide all but 1 of 8 ] Xwmc59-1A Xwmc59-6A Xwmc59.1 Xwmc59.2 wmc59-1A wmc59 wmc59-1B wmc59-6A
Probe for Locus	WMC59
Locus	Xwmc59-1A [ Show all 9 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1e8 Donor: Waugh, SCRI Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 208 bases located on 1A (CA)19 103 to 140. Bands a little weak. Barley - mapped 1HL tip Lina x Canada Park (R. Waugh et al) - at 55C anneal.
Type	PCR SSR
PCR primers	Forward TcATTcgTTgcAgATAcAccAc at 74 length 22 bases, Tm 63 Reverse TcAATgcccTTgTTTcTgAccT at 249 length 22 bases, Tm 65
SSR size	197 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC59
Source Species	Triticum aestivum