GrainGenes Marker Report: Xwmc74-4D
Locus
Xwmc74-4D
Type
Microsatellite
Chromosome
4D
Map
Ta-SSR-2004-4D 84.7
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC74
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc74
[ Show all 8 ]
Probe for Locus
WMC74
Locus
[ Hide all but 1 of 9 ] Xwmc74 Xwmc74b Xwmc74-4D Xwmc74-5A wmc74 wmc74-1A wmc74-1B wmc74-4B wmc74-4D
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6A7 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 234 bases located on 5A, 256 bases located on 4D, 310 bases located on 4B, 5D AGR97. Trimmed to Rsa1 at 3'.(GT)34 190 to 257. (4 polymorphic loci). Related to 1E4
Type
PCR SSR
PCR primers
Forward AAcggcATTgAgcTcAccTTgg at 2 length 22 bases, Tm 69
Reverse TgcgTgAAggcAgcTcAATcgg at 285 length 22 bases, Tm 73
SSR size
305 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC74
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc74-4D
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