GrainGenes Marker Report: Xwmc83-6B
Locus
Xwmc83-6B
Type
Microsatellite
Map
Ta-Synthetic/Opata-GPW-6B -22.8
[ Show Nearby Loci ]
Map Data
Wheat, Synthetic x Opata, GPW
Probe
WMC83
Possible Orthologs
[ Hide all but 1 of 8 ] Xwmc83 Xwmc83-2B Xwmc83-7A Xwmc83a Xwmc83b Xwmc83y wmc83 wmc83-7A
Probe for Locus
WMC83
Locus
Xwmc83-7A
[ Show all 9 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6f8 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 166 bases located on 7A AGR110. Trimmed to insert.(GT)28 32 to 87. (4 polymorphic loci?).
Type
PCR SSR
PCR primers
Forward TggAggAAAcAcAATggATgcc at 7 length 22 bases, Tm 68
Reverse gAgTATcgccgAcgAAAgggAA at 145 length 22 bases, Tm 68
SSR size
160 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC83
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc83-6B
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