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GrainGenes Marker Report: gwm114

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Locus	gwm114
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 3B 209.3 Physical-3B 173.06 Wheat, Yr genes and QTL 3B 98.1
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL
Probe	WMS114
Possible Orthologs	Xgwm114 [ Show all 10 ]
Probe for Locus	WMS114
Locus	[ Hide all but 1 of 11 ] Xgwm114-3A Xgwm114-3B Xgwm114-3D Xgwm114 Xgwm114a Xgwm114b Xgwm114-3B.2 Xgwm114-3B.1 Xgwm114-6R gwm114 gwm114-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)53 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ACAAACAGAAAATCAAAACCCG 3' 5' ATCCATCGCCATTGGAGTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS114
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring