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GrainGenes Marker Report: k00101

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Locus	k00101
Type	CAPS
Chromosome	5H
Map	Hv-HNxH602-5H 131.6
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	baak15n22
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = HhaI SNP = T/G SNP structure (UNICODE) = CGCCTTTGGCKCCCTTGAGAG Parent 1 SNP sequence = CGCCTTTGGCTCCCTTGAGAG Parent 2 SNP sequence = CGCCTTTGGCGCCCTTGAGAG Recognized sequence for CAPs locus = GCGC
Probe for Locus	baak15n22
Locus	k00101
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = unknown protein F1M20.32 [imported] - Arabidopsis thaliana gb AAG52367.1 unknown protein; 89701-90979 [Arabidopsis thaliana]
Type	PCR
PCR primers	Left 5' - GCGTCGAGCACATGAAAGTA Right 5' - GAGGTGGTGTATTGCTCGGT
Sequence	BJ452032
PCR size	352